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Promix is a new type of chromatography stationary phase designed for separation of proteins and peptides. There are four types of phases that SIELC specialists developed for separation of this type of bio-molecule.

is a column for very short and/or very hydrophilic peptides. Typically it is difficult to retain such peptides on RP column. The column offers an improved retention profile and alternative selectivity if compared with RP separation. is a column designed for short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides on this column. Use a buffer with a neutral or slightly acidic pH (4.5 – 7) in the beginning of the gradient, and use a more acidic buffer (pH 2 – 3.5) in the end of the gradient elution. is a column designed for medium size hydrophobic peptides. Typical applications include protein digest which increases peak capacity of the digest by 30-50%. This column offers alternative selectivity if compared with simple RP columns. Pairs of peptides that have been typically difficult to separate, can be resolved on this stationary phase.

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